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DRX527877: CAGE of Jurkat cells activated by CRISPR (chr1:154386334-154387320, technical replicate 1)
1 ILLUMINA (Illumina NovaSeq 6000) run: 66.9M spots, 20.2G bases, 7.1Gb downloads

Submitted by: RIKEN IMS
Study: An atlas of transcribed cis-regulatory elements across human helper T cell diversity
show Abstracthide Abstract
In this study, we present a new ReapTEC approach that simultaneously measures gene expression and enhancer activities using 5' scRNA-seq. By applying ReapTEC to ~500,000 human CD4+ T cells, we generated a multi-modal atlas of heterogenous CD4+ T cells and demonstrate its utility for pinpointing genetic causes of human disease. The Data Access Committee of the National Bioscience Database Center (NBDC) approved that this personal data was made published according to the NBDC Guidelines for Human Data Sharing (https://humandbs.biosciencedbc.jp/en/guidelines/data-sharing-guidelines) as the NBDC Research ID hum0350 and the application ID J-DS000641-001.
Sample: Jurkat cells with CRISPR activation of selected disease-relevant bidirectionally transcribed candidate enhancers (chr1:154386334-154387320) for CAGE
SAMD00766054 • DRS377747 • All experiments • All runs
Organism: Homo sapiens
Library:
Name: CAGE_Jurkat_chr1-154386334-154387320_1
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: CAGE
Layout: PAIRED
Construction protocol: CAGE libraries were generated from total RNA (1 microgram) as previously described (LQ-ssCAGE) with minor modifications.
Pipeline: show...hide...
StepPrev Step
1NIL
Runs: 1 run, 66.9M spots, 20.2G bases, 7.1Gb
Run# of Spots# of BasesSizePublished
DRR54409666,949,46720.2G7.1Gb2024-04-30

ID:
32726822

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